N-terminally His-tagged hepatitis B core antigens: Construction, expression, purification and antigenicity
نویسندگان
چکیده
منابع مشابه
N-terminally His-tagged hepatitis B core antigens: construction, expression, purification and antigenicity.
The core antigen of the hepatitis B virus (HBcAg) has been used widely as a diagnostic reagent for the identification of the viral infection. However, purification using the conventional sucrose density gradient ultracentrifugation is time consuming and costly. To overcome this, HBcAg particles displaying His-tag on their surface were constructed and produced in Escherichia coli. The recombinan...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2009
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2009.04.038